Understanding the Inbreeding & ROH Horse Test
When two related horses are bred together, they can pass down identical stretches of DNA to their offspring. These identical stretches are called ROH (Runs of Homozygosity). Think of ROH as “genetic mirrors”, sections of DNA where both copies (from the sire and the dam) are exactly the same. The longer and more frequent these regions are, the higher the level of inbreeding.
Why Does This Matter? Inbreeding is not always visible in a pedigree. Paper records only show registered ancestry, but they do not reveal how much identical DNA has actually been inherited.
Our advanced Horse Inbreeding SNP-based Test examines thousands of genetic markers across your horse’s genome to measure:
- Total ROH segments: how many identical DNA regions are present.
- Length of ROH regions: longer segments often indicate more recent related matings.
- Genomic Inbreeding Coefficient (GIC): a precise DNA-based percentage of inbreeding.
- Population comparison: how your horse compares to breed reference groups.
The Inbreeding Test provides:
- Measurement of total ROH segments.
- Estimation of genomic inbreeding coefficient (GIC).
- Comparison against population references.
- Objective DNA-based inbreeding assessment.
- Unlike pedigree-based calculations, SNP analysis evaluates the actual genome, not just documented ancestry.
Horse Genetic Diversity (Heterozygosity)
Heterozygosity reflects how genetically varied your horse’s DNA is. The higher diversity generally indicates:
- Broader genetic variability.
- Lower accumulation of identical gene copies.
- Stronger population-level adaptability.
- Reduced diversity may be linked to long-term closed breeding systems.
This analysis helps breeders and owners make informed mating decisions and understand overall genomic structure.
Horse Genetic Identity
The SNP identity profile establishes:
- Unique DNA fingerprint.
- Verification of origin.
- Support in ownership disputes.
- Independent identity confirmation.
This DNA profile remains constant throughout the horse’s lifetime and can serve as a permanent genetic reference.
Benefits of the Horse Inbreeding & Identity Test
The Horse Inbreeding Test & Genetic Identity Test does not screen for specific inherited diseases but evaluates genome-wide structure and identity markers.
- Objective genomic inbreeding measurement.
- Data-driven breeding planning.
- Evaluation of genetic health background.
- Long-term DNA identity record.
- Suitable for sport, breeding and private horses.
Sample Collection of the Horse Inbreeding Test & Genetic Identity Test
Accurate DNA results begin with proper sample collection. For the Horse Inbreeding Test & Genetic Identity Test, DNA is extracted from the follicle (root) attached to plucked mane or tail hairs, not from cut hair. You will need to pull 60–80 hairs, ensuring each strand includes a visible root bulb (white or dark thickened end). The hairs must be securely attached to the sample card using the provided adhesive strip without covering the roots. Each horse must be packaged separately to avoid cross-contamination. No refrigeration is required. Follow the shipping instructions carefully to prevent delays in processing.
- Pull 60 to 80 mane or tail hairs (do not cut them).
- Clearly label the sample card with the required information.
- Place each horse sample in a separate sealable bag.
- Sent the samples.
No veterinary visit is required.
Complete Genomic Background Package (Soon Available)
Upgrade to our exclusive bundle with the Horse Breed Analysis and receive:
- Horse Breed Composition Analysis.
- Inbreeding (ROH & GIC) Evaluation.
- Genetic Diversity Assessment.
- SNP-Based Genetic Identity Profile.
This comprehensive package allows you to:
- Discover breed origins.
- Assess inbreeding and genomic fitness.
- Measure diversity levels.
- Secure a permanent DNA identity profile.
Discover what truly shaped your horse’s genome and gain a deeper understanding of its heritage.
Other Horse DNA Tests at EasyDNA
At EasyDNA, we provide a comprehensive portfolio of equine DNA testing services designed to support breeders, owners, trainers and buyers. From verifying parentage to screening for inherited conditions and establishing a permanent genetic identity, our scientifically validated tests help you make informed, data-driven decisions about your horse’s health, performance and breeding future.
Explore our additional horse DNA testing options below:
- Horse Intolerance Test: Analyse over 300 food and environmental factors to detect potential intolerances and nutritional imbalances.
